Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor
Само за регистроване кориснике
2020
Аутори
Stanković, VesnaĐurđić, Slađana Z.
Ognjanović, Miloš
Antić, Bratislav
Kalcher, Kurt
Mutić, Jelena
Stanković, Dalibor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to th...e convention methods for albumin detection in clinical practice.
Кључне речи:
Electrochemical biosensor / Human serum albumin / Immunosensor / Screen-printed carbon electrodeИзвор:
Journal of Electroanalytical Chemistry, 2020, 860Издавач:
- Elsevier
Финансирање / пројекти:
- Примена унапређених оксидационих процеса и наноструктурисаних оксидних материјала за уклањање загађивача из животне средине, развој и оптимизација инструменталних техника за праћење ефикасности (RS-MESTD-Basic Research (BR or ON)-172030)
- CEEPUS networkCIII-CZ-0212-12-1819-M-120151 (Education of Modern Analytical and Bioanalytical Methods).
- EUREKA project E! 13303 MED-BIO-TEST
Напомена:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/3892
- Peer-reviewed manuscript: http://cherry.chem.bg.ac.rs/handle/123456789/3891
DOI: 10.1016/j.jelechem.2020.113928
ISSN: 1572-6657
WoS: 000519336100030
Scopus: 2-s2.0-85079132277
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Stanković, Vesna AU - Đurđić, Slađana Z. AU - Ognjanović, Miloš AU - Antić, Bratislav AU - Kalcher, Kurt AU - Mutić, Jelena AU - Stanković, Dalibor PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3881 AB - Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice. PB - Elsevier T2 - Journal of Electroanalytical Chemistry T1 - Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor VL - 860 DO - 10.1016/j.jelechem.2020.113928 ER -
@article{ author = "Stanković, Vesna and Đurđić, Slađana Z. and Ognjanović, Miloš and Antić, Bratislav and Kalcher, Kurt and Mutić, Jelena and Stanković, Dalibor", year = "2020", abstract = "Diagnosis and treatment of some important diseased and metabolic disorders is based on successful detection of albumin. In this work, we aim to develop a simple immunosensor for the detection of human serum albumin in biological fluids. Anti-human albumin antibody was covalently attached to the activated surface of screen-printed carbon electrodes enriched with carboxyl graphene/gold nanoparticles composite. Microstructure (TEM, FE-SEM, XRD) and electrochemical (CV, EIS) characterization methods were used to investigate composite properties and to confirm the successful modification of the electrodes. Under the optimal conditions, linear working range and limit of detection were 2.5–500 μg/mL and 1.55 μg/mL, respectively. Additionally, the effect of some possibly interfering compounds was investigated and the immunosensor was used for real sample analysis. The results showed that the sensor exhibited accurate, precise and sensitive characteristics and can be promising replacement to the convention methods for albumin detection in clinical practice.", publisher = "Elsevier", journal = "Journal of Electroanalytical Chemistry", title = "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor", volume = "860", doi = "10.1016/j.jelechem.2020.113928" }
Stanković, V., Đurđić, S. Z., Ognjanović, M., Antić, B., Kalcher, K., Mutić, J.,& Stanković, D.. (2020). Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry Elsevier., 860. https://doi.org/10.1016/j.jelechem.2020.113928
Stanković V, Đurđić SZ, Ognjanović M, Antić B, Kalcher K, Mutić J, Stanković D. Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor. in Journal of Electroanalytical Chemistry. 2020;860. doi:10.1016/j.jelechem.2020.113928 .
Stanković, Vesna, Đurđić, Slađana Z., Ognjanović, Miloš, Antić, Bratislav, Kalcher, Kurt, Mutić, Jelena, Stanković, Dalibor, "Anti-human albumin monoclonal antibody immobilized on EDC-NHS functionalized carboxylic graphene/AuNPs composite as promising electrochemical HSA immunosensor" in Journal of Electroanalytical Chemistry, 860 (2020), https://doi.org/10.1016/j.jelechem.2020.113928 . .