Application of N,N’-Bis(acetylacetonato)propylenediimine Copper(II) Complex as Mediator for Glucose Biosensor
Само за регистроване кориснике
2020
Аутори
Perušković, Danica S.Stevanović, Nikola R.
Kovačević, Gordana
Stanković, Dalibor
Lolić, Aleksandar
Baošić, Rada
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A new glucose biosensor for selective and sensitive determination of glucose in beverage samples was developed. A copper complex with a Schiff base, N,N’-bis(acetylacetonato) propylenediimine, was used as a mediator. It was mixed with glucose oxidase (GOx) and prepared as carbon paste electrode (CPE). Cyclic voltammetry was used for electrochemical measurements. The biosensor showed improved sensitivity when enzyme mediator ratio was 1 : 5, and working pH was 6.6 in 0.1 M phosphate buffer. The linear response of the biosensor for glucose concentration was in the range from 0.5 to 10 mM, with low detection limit of 0.36 mM (calculated as 3 s/n) and sensitivity of 0.16 μA/mM. The reproducibility of the biosensor, calculated as relative standard deviation, was 1.9% for 6 intra-day measurements. Biosensor was stored at +4 °C between experiments, and it was stable during 14 days after the preparation. The developed biosensor was applied on determination of glucose in commercial beverages (a...pple and cherry juice) and obtained results showed good agreement with the reference method.
Кључне речи:
Biosensor / Cyclic Voltammetry / Electrochemistry / Glucose Determination / Schiff Base ComplexИзвор:
ChemistrySelect, 2020, 5, 5, 1671-1675Издавач:
- Wiley-Blackwell
DOI: 10.1002/slct.201904873
ISSN: 2365-6549
WoS: 000526179200010
Scopus: 2-s2.0-85079062956
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Perušković, Danica S. AU - Stevanović, Nikola R. AU - Kovačević, Gordana AU - Stanković, Dalibor AU - Lolić, Aleksandar AU - Baošić, Rada PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/3976 AB - A new glucose biosensor for selective and sensitive determination of glucose in beverage samples was developed. A copper complex with a Schiff base, N,N’-bis(acetylacetonato) propylenediimine, was used as a mediator. It was mixed with glucose oxidase (GOx) and prepared as carbon paste electrode (CPE). Cyclic voltammetry was used for electrochemical measurements. The biosensor showed improved sensitivity when enzyme mediator ratio was 1 : 5, and working pH was 6.6 in 0.1 M phosphate buffer. The linear response of the biosensor for glucose concentration was in the range from 0.5 to 10 mM, with low detection limit of 0.36 mM (calculated as 3 s/n) and sensitivity of 0.16 μA/mM. The reproducibility of the biosensor, calculated as relative standard deviation, was 1.9% for 6 intra-day measurements. Biosensor was stored at +4 °C between experiments, and it was stable during 14 days after the preparation. The developed biosensor was applied on determination of glucose in commercial beverages (apple and cherry juice) and obtained results showed good agreement with the reference method. PB - Wiley-Blackwell T2 - ChemistrySelect T1 - Application of N,N’-Bis(acetylacetonato)propylenediimine Copper(II) Complex as Mediator for Glucose Biosensor VL - 5 IS - 5 SP - 1671 EP - 1675 DO - 10.1002/slct.201904873 ER -
@article{ author = "Perušković, Danica S. and Stevanović, Nikola R. and Kovačević, Gordana and Stanković, Dalibor and Lolić, Aleksandar and Baošić, Rada", year = "2020", abstract = "A new glucose biosensor for selective and sensitive determination of glucose in beverage samples was developed. A copper complex with a Schiff base, N,N’-bis(acetylacetonato) propylenediimine, was used as a mediator. It was mixed with glucose oxidase (GOx) and prepared as carbon paste electrode (CPE). Cyclic voltammetry was used for electrochemical measurements. The biosensor showed improved sensitivity when enzyme mediator ratio was 1 : 5, and working pH was 6.6 in 0.1 M phosphate buffer. The linear response of the biosensor for glucose concentration was in the range from 0.5 to 10 mM, with low detection limit of 0.36 mM (calculated as 3 s/n) and sensitivity of 0.16 μA/mM. The reproducibility of the biosensor, calculated as relative standard deviation, was 1.9% for 6 intra-day measurements. Biosensor was stored at +4 °C between experiments, and it was stable during 14 days after the preparation. The developed biosensor was applied on determination of glucose in commercial beverages (apple and cherry juice) and obtained results showed good agreement with the reference method.", publisher = "Wiley-Blackwell", journal = "ChemistrySelect", title = "Application of N,N’-Bis(acetylacetonato)propylenediimine Copper(II) Complex as Mediator for Glucose Biosensor", volume = "5", number = "5", pages = "1671-1675", doi = "10.1002/slct.201904873" }
Perušković, D. S., Stevanović, N. R., Kovačević, G., Stanković, D., Lolić, A.,& Baošić, R.. (2020). Application of N,N’-Bis(acetylacetonato)propylenediimine Copper(II) Complex as Mediator for Glucose Biosensor. in ChemistrySelect Wiley-Blackwell., 5(5), 1671-1675. https://doi.org/10.1002/slct.201904873
Perušković DS, Stevanović NR, Kovačević G, Stanković D, Lolić A, Baošić R. Application of N,N’-Bis(acetylacetonato)propylenediimine Copper(II) Complex as Mediator for Glucose Biosensor. in ChemistrySelect. 2020;5(5):1671-1675. doi:10.1002/slct.201904873 .
Perušković, Danica S., Stevanović, Nikola R., Kovačević, Gordana, Stanković, Dalibor, Lolić, Aleksandar, Baošić, Rada, "Application of N,N’-Bis(acetylacetonato)propylenediimine Copper(II) Complex as Mediator for Glucose Biosensor" in ChemistrySelect, 5, no. 5 (2020):1671-1675, https://doi.org/10.1002/slct.201904873 . .