Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species
Authorized Users Only
2020
Authors
Dabić Zagorac, DraganaFotirić-Akšić, Milica M.
Glavnik, Vesna
Gašić, Uroš M.
Vovk, Irena
Tešić, Živoslav Lj.
Natić, Maja
Article (Published version)
Metadata
Show full item recordAbstract
The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using thi...s technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.
Keywords:
fingerprint / flavanol / proanthocyanidins / rose hip / thin-layer chromatographySource:
Journal of Separation Science, 2020, 43, 8, 1431-1439Publisher:
- Wiley
Funding / projects:
- Slovenian Research Agency (No. P1-0005).
- ENFIST Centre of Excellence infrastructure (TLC-MS interface).
- The application of new genotypes and technological innovations for the purpose of improvement of fruit-growing and viticultural production (RS-MESTD-Technological Development (TD or TR)-31063)
- Structure-properties relationships of natural and synthetic molecules and their metal complexes (RS-MESTD-Basic Research (BR or ON)-172017)
DOI: 10.1002/jssc.201901271
ISSN: 1615-9306
WoS: 000530019000004
Scopus: 2-s2.0-85079777866
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Dabić Zagorac, Dragana AU - Fotirić-Akšić, Milica M. AU - Glavnik, Vesna AU - Gašić, Uroš M. AU - Vovk, Irena AU - Tešić, Živoslav Lj. AU - Natić, Maja PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4004 AB - The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype. T2 - Journal of Separation Science T1 - Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species VL - 43 IS - 8 SP - 1431 EP - 1439 DO - 10.1002/jssc.201901271 ER -
@article{ author = "Dabić Zagorac, Dragana and Fotirić-Akšić, Milica M. and Glavnik, Vesna and Gašić, Uroš M. and Vovk, Irena and Tešić, Živoslav Lj. and Natić, Maja", year = "2020", abstract = "The profile of flavan-3-ols and proanthocyanidins in five different Rosa species (R. canina, R. glutinosa, R. rubiginosa, R. multiflora, and R. spinosissima) was estimated on high performance thin layer chromatography cellulose plates. Differences in flavanol and proanthocyanidin profiles of the extracts were evident, among which Rosa spinosissima stood out with catechin as the only detected flavanol and red zones as indication of anthocyanins. Furthermore, the elution solvent for thin layer chromatography with mass spectrometry analyses of glycosylated flavan-3-ols and proanthocyanidins was optimized, enabling identification of catechin, (epi)catechin hexoside, proanthocyanidin dimer, and proanthocyanidin dimers and trimers hexosides. A total of 15 flavanols and their derivatives were identified using ultra-high-performance liquid chromatography with linear trap quadrupole-Orbitrap mass analyzer and epicatechin, gallocatechin, and proanthocyanidin trimer were identified only using this technique. However, proanthocyanidin trimer trihexoside was identified only by thin-layer chromatography with mass spectrometry. To establish the relationships between the flavanols and proanthocyanidins composition of rose hip and their origin, principal component analysis was performed on the entire set of liquid chromatography/mass spectrometry data. Both principal components’ scores plots showed that Rosa spinosissima could be considered as an outlier. Our study demonstrated that flavanol and proanthocyanidin profiles of different rose hips depend on the geographical origin rather than on the cultivar and genotype.", journal = "Journal of Separation Science", title = "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species", volume = "43", number = "8", pages = "1431-1439", doi = "10.1002/jssc.201901271" }
Dabić Zagorac, D., Fotirić-Akšić, M. M., Glavnik, V., Gašić, U. M., Vovk, I., Tešić, Ž. Lj.,& Natić, M.. (2020). Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science, 43(8), 1431-1439. https://doi.org/10.1002/jssc.201901271
Dabić Zagorac D, Fotirić-Akšić MM, Glavnik V, Gašić UM, Vovk I, Tešić ŽL, Natić M. Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species. in Journal of Separation Science. 2020;43(8):1431-1439. doi:10.1002/jssc.201901271 .
Dabić Zagorac, Dragana, Fotirić-Akšić, Milica M., Glavnik, Vesna, Gašić, Uroš M., Vovk, Irena, Tešić, Živoslav Lj., Natić, Maja, "Establishing the chromatographic fingerprints of flavan-3-ols and proanthocyanidins from rose hip (Rosa sp.) species" in Journal of Separation Science, 43, no. 8 (2020):1431-1439, https://doi.org/10.1002/jssc.201901271 . .