Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities
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2020
Authors
Pavlović, TamaraDimkić, Ivica
Andrić, Snežana
Milojković-Opsenica, Dušanka
Stanković, Slaviša
Janaćković, Peđa T.
Gavrilović, Milan
Ristivojević, Petar
Article (Published version)
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Linden tea has been used in Serbian folk medicine for centuries to induce sweating for colds, relieve throat irritation and cough, reduce blood pressure, as well as a diuretic, spasmolytic, and as a sedative. The main aim of this study was to determine the comprehensive metabolite profile, free radical scavenging activity, and antimicrobial activities against 23 human and plant pathogens of both commercial and field-collected linden tea samples from Serbia. Developed high-performance thin-layer chromatography (HPTLC) method allowed identification of eight major metabolites in investigated samples, while forty-six metabolites were tentatively identified using high resolution mass spectrometry (HRMS): 14 phenolic acids and their derivatives, 15 glycosides, 9 flavonoids, and 8 procyanidins. Both commercial and field-collected linden tea samples showed similar total phenolic content and radical scavenging activity. Seven compounds such as p-hydroxybenzoic acid 4-O-hexoside, caffeic acid ci...nnamyl ester, pinocembrin, galangin, luteolin 7-O-glucuronide (and its isomer), isorhamnetin hexosyl hexoside were found in Tilia samples for the first time. Radical reagent 2,2-Diphenyl-1-picrylhydrazyl· (DPPH·) was used in DPPH-HPTLC assay which identified chlorogenic acid, caffeic acid, astragalin, quercitrin, tiliroside, and rutin as compounds that exhibit radical scavenging activity. Tilia samples showed inhibitory effect overwhelming only on Gram-positive bacteria, especially on Bacillus subtilis with the lowest MIC values observed, as well as towards Staphylococcus aureus and oral cavity isolates of Streptococcus pyogenes and Streptococcus mutans. Vaginal isolate of Candida glabrata showed more susceptibility than Candida albicans isolate. Generally, extract from Tilia cordata Miller (L4) showed the highest antimicrobial activity against the most of the tested pathogens, among all field-collected Tilia samples.
Keywords:
Antimicrobial activity / HPTLC–UHPLC–HRMS fingerprint / Linden tea from Serbia / Quality assessment / Radical scavenging activitySource:
Industrial Crops and Products, 2020, 154, 112639-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- The Ministry of Education, Science and Technological Development of the Republic of Serbia supported this work Contract number 451-03-68/2020-14/201720
Note:
- Supplementary material: http://cherry.chem.bg.ac.rs/handle/123456789/4041
DOI: 10.1016/j.indcrop.2020.112639
ISSN: 0926-6690
WoS: 000554526900033
Scopus: 2-s2.0-85086395407
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Pavlović, Tamara AU - Dimkić, Ivica AU - Andrić, Snežana AU - Milojković-Opsenica, Dušanka AU - Stanković, Slaviša AU - Janaćković, Peđa T. AU - Gavrilović, Milan AU - Ristivojević, Petar PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4040 AB - Linden tea has been used in Serbian folk medicine for centuries to induce sweating for colds, relieve throat irritation and cough, reduce blood pressure, as well as a diuretic, spasmolytic, and as a sedative. The main aim of this study was to determine the comprehensive metabolite profile, free radical scavenging activity, and antimicrobial activities against 23 human and plant pathogens of both commercial and field-collected linden tea samples from Serbia. Developed high-performance thin-layer chromatography (HPTLC) method allowed identification of eight major metabolites in investigated samples, while forty-six metabolites were tentatively identified using high resolution mass spectrometry (HRMS): 14 phenolic acids and their derivatives, 15 glycosides, 9 flavonoids, and 8 procyanidins. Both commercial and field-collected linden tea samples showed similar total phenolic content and radical scavenging activity. Seven compounds such as p-hydroxybenzoic acid 4-O-hexoside, caffeic acid cinnamyl ester, pinocembrin, galangin, luteolin 7-O-glucuronide (and its isomer), isorhamnetin hexosyl hexoside were found in Tilia samples for the first time. Radical reagent 2,2-Diphenyl-1-picrylhydrazyl· (DPPH·) was used in DPPH-HPTLC assay which identified chlorogenic acid, caffeic acid, astragalin, quercitrin, tiliroside, and rutin as compounds that exhibit radical scavenging activity. Tilia samples showed inhibitory effect overwhelming only on Gram-positive bacteria, especially on Bacillus subtilis with the lowest MIC values observed, as well as towards Staphylococcus aureus and oral cavity isolates of Streptococcus pyogenes and Streptococcus mutans. Vaginal isolate of Candida glabrata showed more susceptibility than Candida albicans isolate. Generally, extract from Tilia cordata Miller (L4) showed the highest antimicrobial activity against the most of the tested pathogens, among all field-collected Tilia samples. PB - Elsevier T2 - Industrial Crops and Products T1 - Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities VL - 154 SP - 112639 DO - 10.1016/j.indcrop.2020.112639 ER -
@article{ author = "Pavlović, Tamara and Dimkić, Ivica and Andrić, Snežana and Milojković-Opsenica, Dušanka and Stanković, Slaviša and Janaćković, Peđa T. and Gavrilović, Milan and Ristivojević, Petar", year = "2020", abstract = "Linden tea has been used in Serbian folk medicine for centuries to induce sweating for colds, relieve throat irritation and cough, reduce blood pressure, as well as a diuretic, spasmolytic, and as a sedative. The main aim of this study was to determine the comprehensive metabolite profile, free radical scavenging activity, and antimicrobial activities against 23 human and plant pathogens of both commercial and field-collected linden tea samples from Serbia. Developed high-performance thin-layer chromatography (HPTLC) method allowed identification of eight major metabolites in investigated samples, while forty-six metabolites were tentatively identified using high resolution mass spectrometry (HRMS): 14 phenolic acids and their derivatives, 15 glycosides, 9 flavonoids, and 8 procyanidins. Both commercial and field-collected linden tea samples showed similar total phenolic content and radical scavenging activity. Seven compounds such as p-hydroxybenzoic acid 4-O-hexoside, caffeic acid cinnamyl ester, pinocembrin, galangin, luteolin 7-O-glucuronide (and its isomer), isorhamnetin hexosyl hexoside were found in Tilia samples for the first time. Radical reagent 2,2-Diphenyl-1-picrylhydrazyl· (DPPH·) was used in DPPH-HPTLC assay which identified chlorogenic acid, caffeic acid, astragalin, quercitrin, tiliroside, and rutin as compounds that exhibit radical scavenging activity. Tilia samples showed inhibitory effect overwhelming only on Gram-positive bacteria, especially on Bacillus subtilis with the lowest MIC values observed, as well as towards Staphylococcus aureus and oral cavity isolates of Streptococcus pyogenes and Streptococcus mutans. Vaginal isolate of Candida glabrata showed more susceptibility than Candida albicans isolate. Generally, extract from Tilia cordata Miller (L4) showed the highest antimicrobial activity against the most of the tested pathogens, among all field-collected Tilia samples.", publisher = "Elsevier", journal = "Industrial Crops and Products", title = "Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities", volume = "154", pages = "112639", doi = "10.1016/j.indcrop.2020.112639" }
Pavlović, T., Dimkić, I., Andrić, S., Milojković-Opsenica, D., Stanković, S., Janaćković, P. T., Gavrilović, M.,& Ristivojević, P.. (2020). Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities. in Industrial Crops and Products Elsevier., 154, 112639. https://doi.org/10.1016/j.indcrop.2020.112639
Pavlović T, Dimkić I, Andrić S, Milojković-Opsenica D, Stanković S, Janaćković PT, Gavrilović M, Ristivojević P. Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities. in Industrial Crops and Products. 2020;154:112639. doi:10.1016/j.indcrop.2020.112639 .
Pavlović, Tamara, Dimkić, Ivica, Andrić, Snežana, Milojković-Opsenica, Dušanka, Stanković, Slaviša, Janaćković, Peđa T., Gavrilović, Milan, Ristivojević, Petar, "Linden tea from Serbia – an insight into the phenolic profile, radical scavenging and antimicrobial activities" in Industrial Crops and Products, 154 (2020):112639, https://doi.org/10.1016/j.indcrop.2020.112639 . .