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Covalent conjugation with (−)-epigallo-catechin 3-gallate and chlorogenic acid changes allergenicity and functional properties of Ara h1 from peanut
dc.creator | He, Weiyi | |
dc.creator | Zhang, Tingting | |
dc.creator | Ćirković-Veličković, Tanja | |
dc.creator | Li, Shuiming | |
dc.creator | Lyu, Yansi | |
dc.creator | Wang, Linlin | |
dc.creator | Yi, Jiang | |
dc.creator | Liu, Zhigang | |
dc.creator | He, Zhendan | |
dc.creator | Wu, Xuli | |
dc.date.accessioned | 2020-09-04T09:52:39Z | |
dc.date.available | 2020-09-04T09:52:39Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0308-8146 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/4053 | |
dc.description.abstract | Ara h1 is a major allergen from peanut. We investigated the effect of covalent conjugation of Ara h1 and dietary polyphenols on allergenicity and functional properties of Ara h1. Enzyme-linked immunosorbent assay revealed that the covalent conjugation of dietary polyphenols significantly reduced the IgE binding capacity of Ara h1. Covalent binding of dietary polyphenols with Ara h1 reduced histamine release by 40% in basophils. The decreased IgE binding capacity of Ara h1 could be ascribed to changes in protein conformation. The IgE epitope of Ara h1 might be blocked by polyphenols at the binding site. Analysis of pepsin digestion of Ara h1–polyphenol conjugates indicated that the covalent binding increased pepsin digestibility and reduced IgE binding capacity. Furthermore, covalent conjugation of Ara h1 with polyphenols decreased denaturation temperature and increased antioxidant activity. Ara h1 conjugated with polyphenols may be a promising approach for reducing the allergenicity of Ara h1. | |
dc.publisher | Elsevier | |
dc.relation | Innovation Project of Universities in Guangdong Province (2018KTSCX192) | |
dc.relation | Key-Area Research and Development Program of Guangdong Province (2019B020213001) | |
dc.relation | Medical Technological Research Funding of Guangdong Province (B2018041) | |
dc.relation | Research Funding of Shenzhen (JCYJ20170818143841444) | |
dc.relation | Research Funding of Shenzhen (JCYJ20180305163606176) | |
dc.rights | restrictedAccess | |
dc.source | Food Chemistry | |
dc.subject | Allergenicity | |
dc.subject | Ara h1 | |
dc.subject | Chlorogenic acid | |
dc.subject | Covalent conjugation | |
dc.subject | EGCG | |
dc.title | Covalent conjugation with (−)-epigallo-catechin 3-gallate and chlorogenic acid changes allergenicity and functional properties of Ara h1 from peanut | |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ли, Схуиминг; Лyу, Yанси; Wанг, Линлин; Yи, Јианг; Ћирковић-Величковић, Тања; Wу, Xули; Хе, Wеиyи; Зханг, Тингтинг; Лиу, Зхиганг; Хе, Зхендан; | |
dc.citation.volume | 331 | |
dc.citation.spage | 127355 | |
dc.identifier.wos | 000559806600003 | |
dc.identifier.doi | 10.1016/j.foodchem.2020.127355 | |
dc.citation.rank | aM21~ | |
dc.type.version | publishedVersion | |
dc.identifier.scopus | 2-s2.0-85086800094 |