Serbian aromatized wine “Bermet”: Electrochemical, chemiluminescent and spectrophotometric determination of antioxidant activity
Autori
Gorjanović, StanislavaPastor, Ferenc
Loupassaki, Sofia
Veljović, Mile S.
Vukosavljević, Predrag V.
Zlatanović, Snežana
Pezo, Lato
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Serbian aromatized wine “Bermet” from grapes grown on Fruška Gora Mountain has been in production since the 15th century. Ten commercial Bermets produced according to the traditional procedure by different manufacturers, and six prepared within the scope of this study were assessed for antioxidant (AO) activity using electrochemical, chemiluminescent and spectrophotometric AO assays. Direct current polarographic assay based on the decrease of anodic current of [hydrogen(peroxido)(1-)]hydroxidomercury(II) complex formation in alkaline H2O2 solution at potential of mercury oxidation, chemiluminescent H2O2 scavenging assay, as well as commonly used spectrophotometric assays (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based Trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP)) were used. Total phenolic content (TPC) was determined by Folin–Ciocalteu assay. The results obtained were correlated usin...g regression analysis, ANOVA and F-test. An integrated approach to AO capacity determination allowed a more comprehensive comparison between samples. The approach is based on the introduction of the relative antioxidant capacity index, calculated by assigning each AO assay equal weight, and by PCA analysis. In addition, the introduction of phenolic antioxidant coefficients, calculated as the ratio between individual AO capacity and TPC, enabled a better understanding of their relation.
Ključne reči:
Antiradical activity / Hydrogen peroxide scavenge / Phenolics / Polarography / Fruška GoraIzvor:
Journal of the Serbian Chemical Society, 2020, 85, 4, 517-529Izdavač:
- Journal of the Serbian Chemical Society
Finansiranje / projekti:
- Molekularne karakteristike kancera (RS-MESTD-MPN2006-2010-143010)
- Uticaj sorte i uslova gajenja na sadržaj bioaktivnih komponenti jagodastog i koštičavog voća i dobijanje biološki vrednih proizvoda poboljšanim i novim tehnologijama (RS-MESTD-Technological Development (TD or TR)-31093)
DOI: 10.2298/JSC190404139G
ISSN: 0352-5139
WoS: 000529068400008
Scopus: 2-s2.0-85086881978
Kolekcije
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Gorjanović, Stanislava AU - Pastor, Ferenc AU - Loupassaki, Sofia AU - Veljović, Mile S. AU - Vukosavljević, Predrag V. AU - Zlatanović, Snežana AU - Pezo, Lato PY - 2020 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4058 AB - Serbian aromatized wine “Bermet” from grapes grown on Fruška Gora Mountain has been in production since the 15th century. Ten commercial Bermets produced according to the traditional procedure by different manufacturers, and six prepared within the scope of this study were assessed for antioxidant (AO) activity using electrochemical, chemiluminescent and spectrophotometric AO assays. Direct current polarographic assay based on the decrease of anodic current of [hydrogen(peroxido)(1-)]hydroxidomercury(II) complex formation in alkaline H2O2 solution at potential of mercury oxidation, chemiluminescent H2O2 scavenging assay, as well as commonly used spectrophotometric assays (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based Trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP)) were used. Total phenolic content (TPC) was determined by Folin–Ciocalteu assay. The results obtained were correlated using regression analysis, ANOVA and F-test. An integrated approach to AO capacity determination allowed a more comprehensive comparison between samples. The approach is based on the introduction of the relative antioxidant capacity index, calculated by assigning each AO assay equal weight, and by PCA analysis. In addition, the introduction of phenolic antioxidant coefficients, calculated as the ratio between individual AO capacity and TPC, enabled a better understanding of their relation. PB - Journal of the Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Serbian aromatized wine “Bermet”: Electrochemical, chemiluminescent and spectrophotometric determination of antioxidant activity VL - 85 IS - 4 SP - 517 EP - 529 DO - 10.2298/JSC190404139G ER -
@article{ author = "Gorjanović, Stanislava and Pastor, Ferenc and Loupassaki, Sofia and Veljović, Mile S. and Vukosavljević, Predrag V. and Zlatanović, Snežana and Pezo, Lato", year = "2020", abstract = "Serbian aromatized wine “Bermet” from grapes grown on Fruška Gora Mountain has been in production since the 15th century. Ten commercial Bermets produced according to the traditional procedure by different manufacturers, and six prepared within the scope of this study were assessed for antioxidant (AO) activity using electrochemical, chemiluminescent and spectrophotometric AO assays. Direct current polarographic assay based on the decrease of anodic current of [hydrogen(peroxido)(1-)]hydroxidomercury(II) complex formation in alkaline H2O2 solution at potential of mercury oxidation, chemiluminescent H2O2 scavenging assay, as well as commonly used spectrophotometric assays (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) based Trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP)) were used. Total phenolic content (TPC) was determined by Folin–Ciocalteu assay. The results obtained were correlated using regression analysis, ANOVA and F-test. An integrated approach to AO capacity determination allowed a more comprehensive comparison between samples. The approach is based on the introduction of the relative antioxidant capacity index, calculated by assigning each AO assay equal weight, and by PCA analysis. In addition, the introduction of phenolic antioxidant coefficients, calculated as the ratio between individual AO capacity and TPC, enabled a better understanding of their relation.", publisher = "Journal of the Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society", title = "Serbian aromatized wine “Bermet”: Electrochemical, chemiluminescent and spectrophotometric determination of antioxidant activity", volume = "85", number = "4", pages = "517-529", doi = "10.2298/JSC190404139G" }
Gorjanović, S., Pastor, F., Loupassaki, S., Veljović, M. S., Vukosavljević, P. V., Zlatanović, S.,& Pezo, L.. (2020). Serbian aromatized wine “Bermet”: Electrochemical, chemiluminescent and spectrophotometric determination of antioxidant activity. in Journal of the Serbian Chemical Society Journal of the Serbian Chemical Society., 85(4), 517-529. https://doi.org/10.2298/JSC190404139G
Gorjanović S, Pastor F, Loupassaki S, Veljović MS, Vukosavljević PV, Zlatanović S, Pezo L. Serbian aromatized wine “Bermet”: Electrochemical, chemiluminescent and spectrophotometric determination of antioxidant activity. in Journal of the Serbian Chemical Society. 2020;85(4):517-529. doi:10.2298/JSC190404139G .
Gorjanović, Stanislava, Pastor, Ferenc, Loupassaki, Sofia, Veljović, Mile S., Vukosavljević, Predrag V., Zlatanović, Snežana, Pezo, Lato, "Serbian aromatized wine “Bermet”: Electrochemical, chemiluminescent and spectrophotometric determination of antioxidant activity" in Journal of the Serbian Chemical Society, 85, no. 4 (2020):517-529, https://doi.org/10.2298/JSC190404139G . .