Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography
Само за регистроване кориснике
2021
Аутори
Lugonja, NikoletaGorjanović, Stanislava
Pastor, Ferenc
Marinković, Vesna S.
Miličić, Biljana
Vrvić, Miroslav M.
Spasić, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol... groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier.
Кључне речи:
Antioxidant / Polarography / Infant Formula / Milk / Proteins / ThiolsИзвор:
Food Analytical Methods, 2021, 14, 1987-1994Издавач:
- Springer Link
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200051 (Институт за општу и физичку хемију, Београд) (RS-MESTD-inst-2020-200051)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200168 (Универзитет у Београду, Хемијски факултет) (RS-MESTD-inst-2020-200168)
DOI: 10.1007/s12161-021-02030-3
ISSN: 1936-9751
WoS: 000640762100001
Scopus: 2-s2.0-85104386895
Колекције
Институција/група
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Lugonja, Nikoleta AU - Gorjanović, Stanislava AU - Pastor, Ferenc AU - Marinković, Vesna S. AU - Miličić, Biljana AU - Vrvić, Miroslav M. AU - Spasić, Snežana PY - 2021 UR - https://cherry.chem.bg.ac.rs/handle/123456789/4517 AB - Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier. PB - Springer Link T2 - Food Analytical Methods T2 - Food Analytical Methods T1 - Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography VL - 14 SP - 1987 EP - 1994 DO - 10.1007/s12161-021-02030-3 ER -
@article{ author = "Lugonja, Nikoleta and Gorjanović, Stanislava and Pastor, Ferenc and Marinković, Vesna S. and Miličić, Biljana and Vrvić, Miroslav M. and Spasić, Snežana", year = "2021", abstract = "Antioxidants present in infant food contribute to its nutritive value and provide protection from oxidative damage. Here, a direct current polarographic method, based on a decrease of anodic current from the hydroxoperhydroxo-mercury(II) complex (HPMC) formed at a mercury dropping electrode at its potential of dissolution, was used to determine the total antioxidant capacity (TAC) of infant formulas, human milk collected from mothers of full-term and preterm infants, human milk supplemented with fortifier, and cow milk. TAC of milk samples and infant formula correlated with protein content measured by the Bradford method (Pearson correlation coefficient 0.605, p<0.001), while TAC of human milk correlated with content of free thiol groups measured by the Ellman method (0.779, p<0.001). The results obtained showed that, in contrast to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, HPMC enables specific insight into the quality of infant milk food which reflects the amounts of free thiol groups and protein content. The significant contribution of milk proteins and the most prevalent amino acids, particularly cysteine, arginine, and phenylalanine, to TAC was shown. Thus, the HPMC method might be useful in monitoring the quality of infant formulas and human milk for preterm infants before and after supplementation with fortifier.", publisher = "Springer Link", journal = "Food Analytical Methods, Food Analytical Methods", title = "Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography", volume = "14", pages = "1987-1994", doi = "10.1007/s12161-021-02030-3" }
Lugonja, N., Gorjanović, S., Pastor, F., Marinković, V. S., Miličić, B., Vrvić, M. M.,& Spasić, S.. (2021). Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography. in Food Analytical Methods Springer Link., 14, 1987-1994. https://doi.org/10.1007/s12161-021-02030-3
Lugonja N, Gorjanović S, Pastor F, Marinković VS, Miličić B, Vrvić MM, Spasić S. Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography. in Food Analytical Methods. 2021;14:1987-1994. doi:10.1007/s12161-021-02030-3 .
Lugonja, Nikoleta, Gorjanović, Stanislava, Pastor, Ferenc, Marinković, Vesna S., Miličić, Biljana, Vrvić, Miroslav M., Spasić, Snežana, "Antioxidant Capacity and Quality of Human Milk and Infant Formula Determined by Direct Current Polarography" in Food Analytical Methods, 14 (2021):1987-1994, https://doi.org/10.1007/s12161-021-02030-3 . .