Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus
Authors
Ljoljić Bilić, VanjaGašić, Uroš M.
Milojković-Opsenica, Dušanka
Rimac, Hrvoje
Vuković Rodriguez, Jadranka
Vlainić, Josipa
Brlek‐Gorski, Diana
Kosalec, Ivan
Article (Published version)
Metadata
Show full item recordAbstract
Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected
to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro
screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that
E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains—
both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental
design consisted of general methods (the serial microdilution broth assay and the agar well diffusion
assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill”
assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial
hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐
guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ
Orbi...Trap MS4. It was determined that active fractions are predominantly formed by gallic acid
derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid
was identified as the most abundant compound. These results point to a direct connection between
galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new
research approaches for future investigation
Keywords:
Erodium cicutarium / MRSA / biofilm / bioautography / fractionation / anti‐hemolytic / phenolic composition / galloyl‐shikimic acidSource:
Аntibiotics, 2022, 11, 492-Publisher:
- MDPI
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.3390/antibiotics11040492
ISSN: 2079-6382
WoS: 00078539660000
Scopus: 2-s2.0-85128467461
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Ljoljić Bilić, Vanja AU - Gašić, Uroš M. AU - Milojković-Opsenica, Dušanka AU - Rimac, Hrvoje AU - Vuković Rodriguez, Jadranka AU - Vlainić, Josipa AU - Brlek‐Gorski, Diana AU - Kosalec, Ivan PY - 2022 UR - http://cherry.chem.bg.ac.rs/handle/123456789/5197 AB - Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains— both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐ guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation PB - MDPI T2 - Аntibiotics T1 - Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus VL - 11 SP - 492 DO - 10.3390/antibiotics11040492 ER -
@article{ author = "Ljoljić Bilić, Vanja and Gašić, Uroš M. and Milojković-Opsenica, Dušanka and Rimac, Hrvoje and Vuković Rodriguez, Jadranka and Vlainić, Josipa and Brlek‐Gorski, Diana and Kosalec, Ivan", year = "2022", abstract = "Followed by a buildup of its phytochemical profile, Erodium cicutarium is being subjected to antimicrobial investigation guided with its ethnobotanical use. The results of performed in vitro screening on Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans strains, show that E. cicutarium has antimicrobial activity, with a particular emphasis on clinical S. aureus strains— both the methicillin sensitive (MSSA) and the methicillin resistant (MRSA) S. aureus. Experimental design consisted of general methods (the serial microdilution broth assay and the agar well diffusion assay), as well as observing bactericidal/bacteriostatic activity through time (the “time‐kill” assay), investigating the effect on cell wall integrity and biofilm formation, and modulation of bacterial hemolysis. Observed antibacterial activity from above‐described methods led to further activity‐ guided fractionation of water and methanol extracts using bioautography coupled with UHPLCLTQ OrbiTrap MS4. It was determined that active fractions are predominantly formed by gallic acid derivatives and flavonol glycosides. Among the most active phytochemicals, galloyl‐shikimic acid was identified as the most abundant compound. These results point to a direct connection between galloyl‐shikimic acid and the observed E. cicutarium antibacterial activity, and open several new research approaches for future investigation", publisher = "MDPI", journal = "Аntibiotics", title = "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus", volume = "11", pages = "492", doi = "10.3390/antibiotics11040492" }
Ljoljić Bilić, V., Gašić, U. M., Milojković-Opsenica, D., Rimac, H., Vuković Rodriguez, J., Vlainić, J., Brlek‐Gorski, D.,& Kosalec, I.. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics MDPI., 11, 492. https://doi.org/10.3390/antibiotics11040492
Ljoljić Bilić V, Gašić UM, Milojković-Opsenica D, Rimac H, Vuković Rodriguez J, Vlainić J, Brlek‐Gorski D, Kosalec I. Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. in Аntibiotics. 2022;11:492. doi:10.3390/antibiotics11040492 .
Ljoljić Bilić, Vanja, Gašić, Uroš M., Milojković-Opsenica, Dušanka, Rimac, Hrvoje, Vuković Rodriguez, Jadranka, Vlainić, Josipa, Brlek‐Gorski, Diana, Kosalec, Ivan, "Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus" in Аntibiotics, 11 (2022):492, https://doi.org/10.3390/antibiotics11040492 . .