The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions
Abstract
Objectives: To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosarninidase (NAG) and NAG isoenzyme activities was studied. Design and methods: After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A(2) forms of NAG were separated by ion-exchange chromatography on DEAE cellulose. Results: A significant decrease in the total activity of NAG in alkaline urines (pH around 8 or higher) was found, which makes this determination unreliable. Analysis of the isoenzymic profiles obtained for weakly acidic and alkaline urines (in the pH range from 5.5. to 10.0) showed that the percent fractions of the individual isoenzyme activities in the total NAG activity and their ratios changed only at pH values above 9.5. Conclusions: The determination of the denoted isoenzymes of urinary NAG after ultrafiltration, dialysis, and chromatographic separation on DEAE cellulose is reliable in a wide range ...of alkaline pH values Of urine. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.
Keywords:
N-acetyl-beta-D-glucosaminidase (NAG) / isoenzymes A, B, and A(2) / isoenzyme stabilities / alkaline conditions / determinationSource:
Clinical Biochemistry, 2005, 38, 4, 384-389Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/j.clinbiochem.2005.01.002
ISSN: 0009-9120
PubMed: 15766740
WoS: 000228116600011
Scopus: 2-s2.0-14844328058
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Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Mandić, Ljuba M. AU - Aćimović, Jelena M. AU - Jovanović, Vesna B. PY - 2005 UR - https://cherry.chem.bg.ac.rs/handle/123456789/692 AB - Objectives: To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosarninidase (NAG) and NAG isoenzyme activities was studied. Design and methods: After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A(2) forms of NAG were separated by ion-exchange chromatography on DEAE cellulose. Results: A significant decrease in the total activity of NAG in alkaline urines (pH around 8 or higher) was found, which makes this determination unreliable. Analysis of the isoenzymic profiles obtained for weakly acidic and alkaline urines (in the pH range from 5.5. to 10.0) showed that the percent fractions of the individual isoenzyme activities in the total NAG activity and their ratios changed only at pH values above 9.5. Conclusions: The determination of the denoted isoenzymes of urinary NAG after ultrafiltration, dialysis, and chromatographic separation on DEAE cellulose is reliable in a wide range of alkaline pH values Of urine. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Clinical Biochemistry T1 - The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions VL - 38 IS - 4 SP - 384 EP - 389 DO - 10.1016/j.clinbiochem.2005.01.002 ER -
@article{ author = "Mandić, Ljuba M. and Aćimović, Jelena M. and Jovanović, Vesna B.", year = "2005", abstract = "Objectives: To have a reliable diagnostic test, the influence of urine pH on the determination of the total activity of N-acetyl-beta-D-glucosarninidase (NAG) and NAG isoenzyme activities was studied. Design and methods: After ultrafiltration and dialysis of the acidic and alkaline urines, the B, A, and A(2) forms of NAG were separated by ion-exchange chromatography on DEAE cellulose. Results: A significant decrease in the total activity of NAG in alkaline urines (pH around 8 or higher) was found, which makes this determination unreliable. Analysis of the isoenzymic profiles obtained for weakly acidic and alkaline urines (in the pH range from 5.5. to 10.0) showed that the percent fractions of the individual isoenzyme activities in the total NAG activity and their ratios changed only at pH values above 9.5. Conclusions: The determination of the denoted isoenzymes of urinary NAG after ultrafiltration, dialysis, and chromatographic separation on DEAE cellulose is reliable in a wide range of alkaline pH values Of urine. (c) 2005 The Canadian Society of Clinical Chemists. All rights reserved.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Clinical Biochemistry", title = "The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions", volume = "38", number = "4", pages = "384-389", doi = "10.1016/j.clinbiochem.2005.01.002" }
Mandić, L. M., Aćimović, J. M.,& Jovanović, V. B.. (2005). The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions. in Clinical Biochemistry Pergamon-Elsevier Science Ltd, Oxford., 38(4), 384-389. https://doi.org/10.1016/j.clinbiochem.2005.01.002
Mandić LM, Aćimović JM, Jovanović VB. The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions. in Clinical Biochemistry. 2005;38(4):384-389. doi:10.1016/j.clinbiochem.2005.01.002 .
Mandić, Ljuba M., Aćimović, Jelena M., Jovanović, Vesna B., "The possibility of determining N-acetyl-beta-D-glucosaminidase isoenzymes under alkaline conditions" in Clinical Biochemistry, 38, no. 4 (2005):384-389, https://doi.org/10.1016/j.clinbiochem.2005.01.002 . .