Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin
Abstract
The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc.
Source:
International Journal of Chemical Kinetics, 2008, 40, 7, 384-394Publisher:
- Wiley-Blackwell, Malden
Funding / projects:
- Istraživanje mehanizma interakcija biološki aktivnih jedinjenja sa biomolekulima (RS-MESTD-MPN2006-2010-142051)
DOI: 10.1002/kin.20319
ISSN: 0538-8066
WoS: 000256689400003
Scopus: 2-s2.0-45749100912
Collections
Institution/Community
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Momic, Tatjana AU - Vujčić, Zoran AU - Vasic, Vesna PY - 2008 UR - https://cherry.chem.bg.ac.rs/handle/123456789/946 AB - The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc. PB - Wiley-Blackwell, Malden T2 - International Journal of Chemical Kinetics T1 - Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin VL - 40 IS - 7 SP - 384 EP - 394 DO - 10.1002/kin.20319 ER -
@article{ author = "Momic, Tatjana and Vujčić, Zoran and Vasic, Vesna", year = "2008", abstract = "The inhibition of myeloperoxidase (MPO), isolated from human neutrophils, by quercetin was investigated by following peroxidase activity of the enzyme using o-dianisidine as the substrate. The inhibition parameters (IC(50)) were obtained by graphical analysis of the inhibition curves. A reaction mechanism, which involved the enzyme inhibition by quercetin and H(2)O(2) in excess, was proposed. The rate and equilibrium constants for the proposed reaction path were calculated from experimental data. Kinetic analysis in noninhibiting H(2)O(2) concentration range in the absence and the presence of quercetin revealed that the reaction mechanism underwent Michaelis-Menten kinetics. K(m)(app,H2O2) and V(max)(app) values indicated that quercetin was a mixed inhibitor of MPO activity. The initial reaction rates were recalculated using the obtained results. Calculated curves fitted the experimental results within the range of experimental error. (C) 2008 Wiley Periodicals, Inc.", publisher = "Wiley-Blackwell, Malden", journal = "International Journal of Chemical Kinetics", title = "Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin", volume = "40", number = "7", pages = "384-394", doi = "10.1002/kin.20319" }
Momic, T., Vujčić, Z.,& Vasic, V.. (2008). Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin. in International Journal of Chemical Kinetics Wiley-Blackwell, Malden., 40(7), 384-394. https://doi.org/10.1002/kin.20319
Momic T, Vujčić Z, Vasic V. Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin. in International Journal of Chemical Kinetics. 2008;40(7):384-394. doi:10.1002/kin.20319 .
Momic, Tatjana, Vujčić, Zoran, Vasic, Vesna, "Kinetics of inhibition of peroxidase activity of myeloperoxidase by quercetin" in International Journal of Chemical Kinetics, 40, no. 7 (2008):384-394, https://doi.org/10.1002/kin.20319 . .