TY  - JOUR
AU  - Apostolović, Danijela
AU  - Grundström, Jeanette
AU  - Kiewiet, Mensiena B. Gea
AU  - Peruško, Marija
AU  - Hamsten, Carl
AU  - Starkhammar, Maria
AU  - Paulie, Staffan
AU  - Hage, Marianne van
PY  - 2023
UR  - http://cherry.chem.bg.ac.rs/handle/123456789/6244
AB  - Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks.
PB  - American Society for Clinical Investigation
T2  - The Journal of Clinical Investigation
T1  - Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome
VL  - 133
IS  - 6
DO  - 10.1172/JCI158357
ER  - 

@article{
author = "Apostolović, Danijela and Grundström, Jeanette and Kiewiet, Mensiena B. Gea and Peruško, Marija and Hamsten, Carl and Starkhammar, Maria and Paulie, Staffan and Hage, Marianne van",
year = "2023",
abstract = "Tick bites have been shown to transmit a novel form of severe food allergy, the galactose-α-1,3-galactose (α-Gal) syndrome (AGS). Cellular responses to α-Gal in patients with AGS have, to date, not been thoroughly scrutinized. Therefore, we investigated T and B cell proliferation, activation, and cytokine profiles in response to tick protein extract (TE) and α-Gal-free TE in patients with AGS and in healthy controls. T and B cells from both patients and controls proliferated in response to TE, but significantly more in patients with AGS. B cell proliferation, but not T cell proliferation, in patients with AGS was reduced by removing α-Gal from the TE. In addition, TE induced a clear Th2 cytokine profile in patients with AGS. Expression of CD23 by B cells correlated only to T cell proliferation. However, both B cell proliferation and CD23 expression were reduced when CD40L and IL-4 were blocked. A large portion of the IgG1 and IgE antibodies binding TE in patients with AGS were directed against the α-Gal epitope. We have, for what we believe to be the first time, investigated T and B cell responses to α-Gal carrying tick proteins in patients with AGS, which will be essential for the understanding of the immune response against an allergenic carbohydrate transmitted by ticks.",
publisher = "American Society for Clinical Investigation",
journal = "The Journal of Clinical Investigation",
title = "Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome",
volume = "133",
number = "6",
doi = "10.1172/JCI158357"
}

Apostolović, D., Grundström, J., Kiewiet, M. B. G., Peruško, M., Hamsten, C., Starkhammar, M., Paulie, S.,& Hage, M. v.. (2023). Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome. in The Journal of Clinical Investigation
American Society for Clinical Investigation., 133(6).
https://doi.org/10.1172/JCI158357

Apostolović D, Grundström J, Kiewiet MBG, Peruško M, Hamsten C, Starkhammar M, Paulie S, Hage MV. Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome. in The Journal of Clinical Investigation. 2023;133(6).
doi:10.1172/JCI158357 .

Apostolović, Danijela, Grundström, Jeanette, Kiewiet, Mensiena B. Gea, Peruško, Marija, Hamsten, Carl, Starkhammar, Maria, Paulie, Staffan, Hage, Marianne van, "Th2-skewed T cells correlate with B cell response to α-Gal and tick antigens in α-Gal syndrome" in The Journal of Clinical Investigation, 133, no. 6 (2023),
https://doi.org/10.1172/JCI158357 . .