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dc.creatorTripkovic, T.
dc.creatorCharvy, C.
dc.creatorAlves, S.
dc.creatorLolić, Aleksandar
dc.creatorBaošić, Rada
dc.creatorNikolić-Mandić, Snezana D.
dc.creatorTabet, J. C.
dc.date.accessioned2018-11-22T00:23:23Z
dc.date.available2018-11-22T00:23:23Z
dc.date.issued2013
dc.identifier.issn0039-9140
dc.identifier.urihttp://cherry.chem.bg.ac.rs/handle/123456789/1366
dc.description.abstractAim of this work is to propose an analytical protocol for proteinaceous binder identification in paintings using tryptic peptide analysis and MALDI-TOF mass spectrometry strengthened with MALDI-TOF/TOF tandem mass spectrometry (LIFT method). Proteinaceous binders are enzymatically digested with trypsin. From each individual protein frequently occurring in binders, a specific set of peptides is releasing during enzymatic digestion giving a peptide mass fingerprint (PMF) of that particular protein. The most intensive peptide peaks in PMF were determined and annotated with their corresponding amino acid sequence by MALDI-TOF/TOF analysis and subsequent database search. Before analyzing historical painting samples, procedure was tested and optimized on several painting model samples for a reliable and efficient identification of proteinaceous materials. The method is avoiding sample manipulation as much as possible in order to reduce sample loss. Since the applied procedures led to protein identification of binding media in model samples, MALDI-TOF/TOF was for the first time applied for analysis of proteinaceous binders in old painting samples. (C) 2013 Elsevier B.V. All rights reserved.en
dc.publisherElsevier Science Bv, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172051/RS//
dc.rightsrestrictedAccess
dc.sourceTalanta
dc.subjectMALDI TOF MSen
dc.subjectLIFT-TOF/TOF tandem mass spectrometryen
dc.subjectPeptide mass fingerprinten
dc.subjectProteinaceous bindersen
dc.subjectHistorical paintingsen
dc.titleIdentification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometryen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБаошић, Рада; Лолић, Aлександар; Aлвес, С.; Николиц-Мандиц, С. Д.; Трипковиц, Т.; Табет, Ј. Ц.; Цхарвy, Ц.;
dc.citation.volume113
dc.citation.spage49
dc.citation.epage61
dc.identifier.wos000320416500009
dc.identifier.doi10.1016/j.talanta.2013.03.071
dc.citation.other113: 49-61
dc.citation.rankM21
dc.identifier.pmid23708623
dc.type.versionpublishedVersionen
dc.identifier.scopus2-s2.0-84876786177
dc.identifier.rcubKon_2486


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