Development and validation of a simple thin-layer chromatographic method for the analysis of p-chlorophenol in treated wastewater

Abstract

A thin-layer chromatographic (TLC) method with densitometric detection was established for the quantification of p-chlorophenol in wastewater. Degradation efficiency of p-chlorophenol was monitored after each treatment of the wastewater samples. Degradation of p-chlorophenol was performed by advanced oxidation processes (AOPs), using UV, H2O2/UV, O-3/H2O2/UV, O-3 and O-3/UV. The developed TLC procedure was found to be simple, rapid and precise. The method is characterized by high sensitivity (the limit of detection was 11 ng per band and limit of quantification 35 ng per band), a linear range from 75 to 500 ng per band, r = 0.9965), and high precision, accuracy (mean percentage recovery 98.6 %), and specificity. Additionally, the efficiency of degradation was monitored using HPLC giving comparable results with the reversed phase TLC measurements.

Kvantifikacija p-hlorfenola u uzorcima otpadnih voda urađena je primenom tankoslojne hromatografije sa denzitometrijskom detekcijom. Efikasnost degradacije p-hlorfenola praćena je nakon svakog tretmana uzorka otpadne vode. UV svetlost, O3, kao i kombinacija H2O2/UV, O3/H2O2/UV i O3/UV su korišćeni za degradaciju p-hlorfenola. Primenjen TLC postupak je jednostavan, brz i precizan. Visoka osetljivost (limit detekcije je 11 ng po traci i limit kvantifikacije je 35 ng po traci), opseg linearnosti (od 75 do 500 ng po traci, r = 0,9965), visoka preciznost i tačnost, kao i specifičnost su karakteristike korišćenog TLC postupka. HPLC metoda, kao standardna metoda za određivanje p-hlorfenola je takođe primenjena za proučavanje efikasnosti postupka degradacije. Rezultati dobijeni primenom TLC metode su uporedivi sa HPLC merenjima.