Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization
Samo za registrovane korisnike
2013
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color. (C) 2013 Elsevier Ltd. All rights reserved.
Ključne reči:
Laccase / Bacillus amyloliquefaciens / Decolorization / BiodegradationIzvor:
Bioresource Technology, 2013, 147, 177-183Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-MESTD-Basic Research (BR or ON)-172048)
- Joint Serbian-Spanish Action
- Spanish MICINN [CTQ2011-28398-CO2-01]
DOI: 10.1016/j.biortech.2013.08.056
ISSN: 0960-8524
PubMed: 23994699
WoS: 000326660900026
Scopus: 2-s2.0-84883380664
Institucija/grupa
Hemijski fakultet / Faculty of ChemistryTY - JOUR AU - Lončar, Nikola L. AU - Božić, Nataša AU - Lopez-Santin, Josep AU - Vujčić, Zoran PY - 2013 UR - https://cherry.chem.bg.ac.rs/handle/123456789/1431 AB - One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color. (C) 2013 Elsevier Ltd. All rights reserved. PB - Elsevier Sci Ltd, Oxford T2 - Bioresource Technology T1 - Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization VL - 147 SP - 177 EP - 183 DO - 10.1016/j.biortech.2013.08.056 ER -
@article{ author = "Lončar, Nikola L. and Božić, Nataša and Lopez-Santin, Josep and Vujčić, Zoran", year = "2013", abstract = "One hundred wild type strains of Bacillus sp. were isolated from industrial and agricultural soil across Serbia and screened for laccase activity. Three strains showed high laccase activity temperature optimum of 65 and 80 degrees C towards ABTS. A new laccase gene from the strain with highest temperature optimum, namely Bacillus amyloliquefaciens 12B was cloned and expressed in Escherichia coli. Recombinant laccase degraded dye Reactive blue 52 at pH 7.0 and pH 4.0 and at elevated temperature, while fungal laccases was unable to act on this substrate at pH higher than 4.0 and was quickly inactivated at temperatures higher than 45 degrees C. Degradation of dye was monitored by HPLC-DAD and resulting precipitate was analyzed by FTIR spectroscopy. Single product peak without chromophore was detected in solution, while water insoluble aggregate, presumably dye polymer is formed retaining blue color. (C) 2013 Elsevier Ltd. All rights reserved.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Bioresource Technology", title = "Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization", volume = "147", pages = "177-183", doi = "10.1016/j.biortech.2013.08.056" }
Lončar, N. L., Božić, N., Lopez-Santin, J.,& Vujčić, Z.. (2013). Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization. in Bioresource Technology Elsevier Sci Ltd, Oxford., 147, 177-183. https://doi.org/10.1016/j.biortech.2013.08.056
Lončar NL, Božić N, Lopez-Santin J, Vujčić Z. Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization. in Bioresource Technology. 2013;147:177-183. doi:10.1016/j.biortech.2013.08.056 .
Lončar, Nikola L., Božić, Nataša, Lopez-Santin, Josep, Vujčić, Zoran, "Bacillus amyloliquefaciens laccase - From soil bacteria to recombinant enzyme for wastewater decolorization" in Bioresource Technology, 147 (2013):177-183, https://doi.org/10.1016/j.biortech.2013.08.056 . .