A progressive electrochemical sensor for food quality control: Reliable determination of theobromine in chocolate products using a miniaturized boron-doped diamond electrode
Само за регистроване кориснике
2018
Аутори
Šovrc, ĽubomírHasso, Marek
Sarakhman, Olha
Kianicková, Kristína
Stanković, Dalibor
Otrisal, Pavel
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In the present paper, a miniaturized boron-doped diamond electrode (BDDE) is proposed as a progressive electrochemical sensor for simple, fast and reliable quantification of dimethylxanthine alkaloid, theobromine (TB). Using cyclic voltammetry, the studied xanthine provided one well-shaped, irreversible and diffusion-controlled oxidation peak at relatively high potentials (+ 1.2 V vs. Ag/AgCl/3 M KCl reference electrode) in the presence of 0.1 M H2SO4. After selection of suitable experimental conditions, the linear calibration curves for TB were obtained in the concentration range from 0.99 up to 54.5 mu M with the sensitivity of 0.07 mu A/mu M providing both differential pulse (DPV) and square-wave voltammetric (SWV) techniques, respectively. The elaborated voltammetric protocol yielded low detection limits of 0.42 and 0.51 mu M accompanied by adequate intra-day repeatability (relative standard deviation of 2.5 and 1.7%) using DPV and SWV procedure, respectively. The interference stud...y revealed the reasonable selectivity when taking the target food samples into account. The practical applicability of the voltammetric protocol using a miniaturized BDDE was verified in the analysis of six commercially available brands of chocolate products with the determined mass percentages of TB ranging from 0.75 to 2.24% and from 0.69 to 2.15% using DPV and SWV procedure, respectively. The obtained results were also in a good agreement with those achieved by reference titration method with potentiometric indication. The progressive electrochemical sensor based on a miniaturized BDDE has appeared to be an attractive candidate for practical applications in food quality control. Besides, the proposed voltammetric protocol presents advantages when compared to others techniques (e.g. chromatography), concerning simplicity, cost, speed of analysis, waste generation (environmentally friendly) and samples pretreatment (only dilution in electrolyte solution prior to analysis).
Кључне речи:
Dimethylxanthine alkaloid / Boron-doped diamond electrode / Electrochemical sensor / Voltammetric techniques / Real sample analysisИзвор:
Microchemical Journal, 2018, 142, 297-304Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Примена унапређених оксидационих процеса и наноструктурисаних оксидних материјала за уклањање загађивача из животне средине, развој и оптимизација инструменталних техника за праћење ефикасности (RS-MESTD-Basic Research (BR or ON)-172030)
- Strengthening of the MagBioVin Research and Innovation Team for Development of Novel Approaches for Tumour Therapy based on Nanostructured Materials (EU-FP7-621375)
- Grant Agency of the Slovak Republic [1/0489/16]
DOI: 10.1016/j.microc.2018.07.007
ISSN: 0026-265X
WoS: 000442708800039
Scopus: 2-s2.0-85049787620
Колекције
Институција/група
Inovacioni centar / Innovation CentreTY - JOUR AU - Šovrc, Ľubomír AU - Hasso, Marek AU - Sarakhman, Olha AU - Kianicková, Kristína AU - Stanković, Dalibor AU - Otrisal, Pavel PY - 2018 UR - https://cherry.chem.bg.ac.rs/handle/123456789/2208 AB - In the present paper, a miniaturized boron-doped diamond electrode (BDDE) is proposed as a progressive electrochemical sensor for simple, fast and reliable quantification of dimethylxanthine alkaloid, theobromine (TB). Using cyclic voltammetry, the studied xanthine provided one well-shaped, irreversible and diffusion-controlled oxidation peak at relatively high potentials (+ 1.2 V vs. Ag/AgCl/3 M KCl reference electrode) in the presence of 0.1 M H2SO4. After selection of suitable experimental conditions, the linear calibration curves for TB were obtained in the concentration range from 0.99 up to 54.5 mu M with the sensitivity of 0.07 mu A/mu M providing both differential pulse (DPV) and square-wave voltammetric (SWV) techniques, respectively. The elaborated voltammetric protocol yielded low detection limits of 0.42 and 0.51 mu M accompanied by adequate intra-day repeatability (relative standard deviation of 2.5 and 1.7%) using DPV and SWV procedure, respectively. The interference study revealed the reasonable selectivity when taking the target food samples into account. The practical applicability of the voltammetric protocol using a miniaturized BDDE was verified in the analysis of six commercially available brands of chocolate products with the determined mass percentages of TB ranging from 0.75 to 2.24% and from 0.69 to 2.15% using DPV and SWV procedure, respectively. The obtained results were also in a good agreement with those achieved by reference titration method with potentiometric indication. The progressive electrochemical sensor based on a miniaturized BDDE has appeared to be an attractive candidate for practical applications in food quality control. Besides, the proposed voltammetric protocol presents advantages when compared to others techniques (e.g. chromatography), concerning simplicity, cost, speed of analysis, waste generation (environmentally friendly) and samples pretreatment (only dilution in electrolyte solution prior to analysis). PB - Elsevier Science Bv, Amsterdam T2 - Microchemical Journal T1 - A progressive electrochemical sensor for food quality control: Reliable determination of theobromine in chocolate products using a miniaturized boron-doped diamond electrode VL - 142 SP - 297 EP - 304 DO - 10.1016/j.microc.2018.07.007 ER -
@article{ author = "Šovrc, Ľubomír and Hasso, Marek and Sarakhman, Olha and Kianicková, Kristína and Stanković, Dalibor and Otrisal, Pavel", year = "2018", abstract = "In the present paper, a miniaturized boron-doped diamond electrode (BDDE) is proposed as a progressive electrochemical sensor for simple, fast and reliable quantification of dimethylxanthine alkaloid, theobromine (TB). Using cyclic voltammetry, the studied xanthine provided one well-shaped, irreversible and diffusion-controlled oxidation peak at relatively high potentials (+ 1.2 V vs. Ag/AgCl/3 M KCl reference electrode) in the presence of 0.1 M H2SO4. After selection of suitable experimental conditions, the linear calibration curves for TB were obtained in the concentration range from 0.99 up to 54.5 mu M with the sensitivity of 0.07 mu A/mu M providing both differential pulse (DPV) and square-wave voltammetric (SWV) techniques, respectively. The elaborated voltammetric protocol yielded low detection limits of 0.42 and 0.51 mu M accompanied by adequate intra-day repeatability (relative standard deviation of 2.5 and 1.7%) using DPV and SWV procedure, respectively. The interference study revealed the reasonable selectivity when taking the target food samples into account. The practical applicability of the voltammetric protocol using a miniaturized BDDE was verified in the analysis of six commercially available brands of chocolate products with the determined mass percentages of TB ranging from 0.75 to 2.24% and from 0.69 to 2.15% using DPV and SWV procedure, respectively. The obtained results were also in a good agreement with those achieved by reference titration method with potentiometric indication. The progressive electrochemical sensor based on a miniaturized BDDE has appeared to be an attractive candidate for practical applications in food quality control. Besides, the proposed voltammetric protocol presents advantages when compared to others techniques (e.g. chromatography), concerning simplicity, cost, speed of analysis, waste generation (environmentally friendly) and samples pretreatment (only dilution in electrolyte solution prior to analysis).", publisher = "Elsevier Science Bv, Amsterdam", journal = "Microchemical Journal", title = "A progressive electrochemical sensor for food quality control: Reliable determination of theobromine in chocolate products using a miniaturized boron-doped diamond electrode", volume = "142", pages = "297-304", doi = "10.1016/j.microc.2018.07.007" }
Šovrc, Ľ., Hasso, M., Sarakhman, O., Kianicková, K., Stanković, D.,& Otrisal, P.. (2018). A progressive electrochemical sensor for food quality control: Reliable determination of theobromine in chocolate products using a miniaturized boron-doped diamond electrode. in Microchemical Journal Elsevier Science Bv, Amsterdam., 142, 297-304. https://doi.org/10.1016/j.microc.2018.07.007
Šovrc Ľ, Hasso M, Sarakhman O, Kianicková K, Stanković D, Otrisal P. A progressive electrochemical sensor for food quality control: Reliable determination of theobromine in chocolate products using a miniaturized boron-doped diamond electrode. in Microchemical Journal. 2018;142:297-304. doi:10.1016/j.microc.2018.07.007 .
Šovrc, Ľubomír, Hasso, Marek, Sarakhman, Olha, Kianicková, Kristína, Stanković, Dalibor, Otrisal, Pavel, "A progressive electrochemical sensor for food quality control: Reliable determination of theobromine in chocolate products using a miniaturized boron-doped diamond electrode" in Microchemical Journal, 142 (2018):297-304, https://doi.org/10.1016/j.microc.2018.07.007 . .