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The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate
dc.creator | Nikodinović-Runić, Jasmina | |
dc.creator | Kenny, Shane T. | |
dc.creator | Babu, Ramesh P. | |
dc.creator | Woods, Trevor | |
dc.creator | Blau, Werner J. | |
dc.creator | O'Connor, Kevin E. | |
dc.date.accessioned | 2018-11-22T00:13:32Z | |
dc.date.available | 2018-11-22T00:13:32Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 0175-7598 | |
dc.identifier.uri | https://cherry.chem.bg.ac.rs/handle/123456789/967 | |
dc.description.abstract | Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C. | en |
dc.publisher | Springer, New York | |
dc.rights | restrictedAccess | |
dc.source | Applied Microbiology and Biotechnology | |
dc.title | The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Кеннy, Схане Т.; Никодиновић-Рунић, Јасмина; Блау, Wернер Ј.; О'Цоннор, Кевин Е.; Бабу, Рамесх П.; Wоодс, Тревор; | |
dc.citation.volume | 80 | |
dc.citation.issue | 4 | |
dc.citation.spage | 665 | |
dc.citation.epage | 673 | |
dc.identifier.wos | 000258881400013 | |
dc.identifier.doi | 10.1007/s00253-008-1593-0 | |
dc.citation.other | 80(4): 665-673 | |
dc.citation.rank | M22 | |
dc.identifier.pmid | 18629491 | |
dc.type.version | publishedVersion | en |
dc.identifier.scopus | 2-s2.0-50849084452 |